DR2 INRA, UMR1347 Agroécologie, GEAPSI group. Co-leader of the FILEAS team (Seed FIlling & Abiotic Stresses) whose project is mainly dedicated to the optimization and stabilization of seed quality components in European grain legume crops, such as pea. We study molecular processes controlling the establishment of seed quality, notably those related to the remobilization of nutrients from source organs to seeds. We investigate the effect of water deficit and sulfur deficiency, two stresses that interact in the current context of climate change and low atmospheric sulfur deposition, in view of identifying genes that might help legume plants to maintain seed yield, protein content and composition under such adverse conditions

My research project focuses on the understanding of the molecular mechanisms controlling seed composition and its plasticity under environmental constraints, mainly sulfur deficiency, with the objective of optimizing and stabilizing the nutritional value of grain legume seeds. This project is structured into two axes. The
consists in using the model legume species Medicago truncatula to boost the identification of genes controlling seed development and then in transferring the information to important legume crops, in particular pea for which extensive genomics and post-genomics resources are now available. The second focuses on sulfur nutrition and its role in the pea response to water deficit (thesis of Charlotte Henriet). These two axes target the optimization and stabilization of seed quality (e.g. size and protein composition) and are strongly connected owing to the importance of sulfur nutrition in the establishment of seed quality.

M1 Sciences de la Vie et de la Santé and M2R B2IPME Biologie Intégrative des Interactions Plante Microbe Environnement (Université de Bourgogne Franche-Comté)

Articles de recherche:

• Maillard A, Sorin E, Etienne P, Diquélou S, Koprivova A, Kopriva S, Arkoun M, Gallardo K, Turner M, Cruz F, Yvin JC, Ourry A (2016) Non-Specific Root Transport of Nutrient Gives Access to an Early Nutritional Indicator: The Case of Sulfate and Molybdate. PLoS One. 11(11): e0166910.
• Satgé C, Moreau S, Sallet E, Lefort G, Auriac MC, Remblière C, Cottret L, Gallardo K, Noirot C, Jardinaud MF, Gamas P (2016) Reprogramming of DNA methylation is critical for nodule development in Medicago truncatula. Nat Plants 2(11): 16166.
• Zinsmeister J, Lalanne D, Terrasson E, Chatelain E, Vandecasteele C, Vu BL, Dubois-Laurent C, Geoffriau E, Signor CL, Dalmais M, Gutbrod K, Dörmann P, Gallardo K, Bendahmane A, Buitink J, Leprince O (2016) ABI5 Is a Regulator of Seed Maturation and Longevity in Legumes. Plant Cell. 28: 2735-2754.
• Noguero M, Le Signor C, Vernoud V, Bandyopadhyay K, Sanchez M, Fu V, Torres-Jerez I, Wen J, Mysore KS, Gallardo K, Udvardi M, Thompson R, Verdier J (2015) DASH transcription factor impacts seed size by its action on embryo morphogenesis and auxin homeostasis. Plant Journal 81: 453-66. Les deux premiers auteurs ont participé de façon équivalente à ce travail. Classification Revue Q1 "Plant sciences" IF 6.
• Brosowska-Arendt W, Gallardo K, Sommerer N, Weidner S (2014) Changes in the proteome of pea (Pisum sativum L.) seeds germinating under optimal and osmotic stress conditions and subjected to post-stress recovery. Journal of Proteomics. Acta Physiologiae Plantarum 36: 795-807. Classification Revue Q1 "Plant sciences" IF 1.6.
• D'Hooghe P, Dubousset L, Gallardo K, Kopriva S, Avice J-C, Trouverie J (2014) Evidence for proteomic and metabolic adaptations associated to alterations of seed yield and quality in sulphur-limited Brassica napus L. Molecular & Cellular Proteomics, 13: 1165-1183. Classification Revue Q1 “Biochemical Research Methods” IF 6.5.
• Gallardo K, Courty PE, Le Signor C, Wipf D, Vernoud V (2014) Sulfate transporters in the plant's response to drought and salinity: regulation and possible functions. Frontiers in Plant Science 5: 580. Review. Classification Revue Q1 "Plant sciences" IF3.9.
• Salon C, Bataillé MP, Gallardo K, Jeudy C, Santoni AL, Trouverie J, Voisin AS, Avice JC (2014) (34)S and (15)N labelling to model S and N flux in plants and determine the different components of N and S use efficiency. Methods in Molecular Biology 1090: 335-46.
• Zuber H, Poignavent G, Le Signor C, Aimé D, Vieren E, Tadla C, Belghazi M, Labas V, Santoni A-L, Wipf D, Buitink J, Avice J-C, Salon C, Gallardo K (2013) Legume adaptation to sulfur deficiency revealed by comparing nutrient allocation and seed traits in Medicago truncatula. Plant Journal 76: 982-96. Les deux premiers auteurs ont participé de façon équivalente à ce travail. Classification Revue Q1 "Plant sciences" IF 6.
• Casieri L, Gallardo K, Wipf D (2012) Transcriptional response of Medicago truncatula sulphate transporters to arbuscular mycorrhizal symbiosis with and without sulphur stress. Planta 235: 1431-4. Classification Revue Q1 “Plant Sciences” IF 3.3.
• D'Erfurth I, Le Signor C, Aubert G, Sanchez M, Vernoud V, Darchy B, Lherminier J, Bourion V, Bouteiller N, Bendahmane A, Buitink J, Prosperi JM, Thompson R, Burstin J, Gallardo K (2012) A role for an endosperm-localized subtilase in the control of seed size in legumes. New Phytologist 196: 738-51. Les deux premiers auteurs ont participé de façon équivalente à ce travail. Classification Revue Q1 “Plant Sciences” IF 7.7.
• Rajjou L, Duval M, Gallardo K, Catusse J, Bally J, Job C, Job D (2012). Seed germination and vigor. Annual Review of Plant Biololgy 63:507-533. Classification Revue Q1 "Plant sciences" IF 23.3.
• Rajjou L, Belghazi M, Catusse J, Ogé L, Arc E, Godin B, Chibani K, Ali-Rachidi S, Collet B, Grappin P, Jullien M, Gallardo K, Job C, Job D (2011) Proteomics and posttranslational proteomics of seed dormancy and germination. Methods in Molecular Biology 773: 215-36.
• Repetto O, Rogniaux H, Larre C, Thompson R, Gallardo K (2012). The seed nuclear proteome. Frontiers in Plant Science 3:289. Review. Classification Revue Q1 "Plant sciences" IF 3.9.
• Vandecasteele C, Teulat-Merah B, Morère-Le Paven MC, Leprince O, Ly Vu B, Viau L, Ledroit L, Pelletier S, Payet N, Satour P, Lebras C, Gallardo K, Huguet T, Limami AM, Prosperi JM, Buitink J (2011) QTL analysis reveals a correlation between the ratio of sucrose/raffinose family oligosaccharides and seed vigour in Medicago truncatula. Plant, Cell and Environment 34: 1473–1487. Classification Revue Q1 “Plant Sciences » IF 5.145
• Zuber H, Davidian JC, Aimé D, Belghazi M, Wirtz M, Hell R, Thompson R and Gallardo K (2010a) The seed composition of Arabidopsis mutants for the group 3 sulphate transporters indicates a role in sulphate translocation within developing seeds. Plant Physiology, 154: 913-926. Classification Revue Q1 “Plant Sciences” IF 7.089
• Zuber H, Davidian JC, Wirtz M, Hell R, Belghazi M, Thompson R and Gallardo K (2010b). Sultr4;1 mutant seeds of Arabidopsis have an enhanced sulphate content and modified proteome suggesting metabolic adaptations to altered sulphate compartmentalization. BMC Plant Biology 10: 78. Classification Revue Q1 “Plant Sciences” IF 4.77
• Rispail N, Kaló P, Kiss G, Ellis N, Gallardo K, Thompson R.D., Prats E, Larrainzar E, Ladrera R, González E, Arrese-Igor C, Ferguson B, Gresshoff P, Rubiales D (2010) Model legumes contribute to faba bean breeding. Field Crop Research 115, 253-269. Classification Revue Q1 "Agronomy" IF 2.989
• Thompson R, Burstin J, Gallardo K (2009) Post-genomics studies of developmental processes in legume seeds. Plant Physiol. 151: 1023-1029. Classification Revue Q1 "Plant Sciences" IF 7.089
• Gallardo K, Thompson R, Burstin J (2008) Reserve accumulation in legume seeds. Comptes Rendus Biologies 331: 755-762. Classification Revue Q2 "Biology" IF 1.915
• Repetto O, Rogniaux H, Firnhaber C, Zuber H*, Küster H, Larré C, Thompson R, Gallardo K (2008) Exploring the nuclear proteome of M. truncatula at the switch towards seed filling. Plant Journal 56: 398-410. Classification Revue Q1 “Plant sciences” IF 7.113
• Verdier J, Kakar K, Gallardo K, Le Signor C, Aubert G, Schlereth A, Town C, Udvardi M, Thompson R (2008) Gene expression profiling of M. truncatula transcription factors identifies putative regulators of grain legume seed filling. Plant Molecular Biology 67: 567-580. Classification Revue Q1 “Plant Sciences” IF 4.398
• Gallardo K, Firnhaber C, Zuber H, Hericher D, Belghazi M, Henry C, Küster H and Thompson R (2007) A combined proteome and transcriptome analysis of developing M. truncatula seeds: evidence for metabolic specialization of maternal and filial tissues. Molecular & Cellular Proteomics 6.12: 2165-2179. Classification Revue Q1 “Biochemical Research Methods” IF 8.051
• Domoney C, Duc G, Ellis N, Ferrandiz C, Firnhaber C, Gallardo K, Hofer J, Kopka J, Küster H, Madueno F, Munier-Jolain N, Mayer K, Thompson R, Udvardi M and Salon C (2006) Genetic and genomic analysis of legume flowers and seeds. Current Opinion in Plant Biology 9: 133-141. Classification revue Q1 "Plant Sciences" IF 9,221
• Gallardo K, Kurt C, Thompson R, Ochatt S (2006) In vitro culture of immature M. truncatula grains under conditions permitting embryo development comparable to that observed in vivo. Plant Science 170: 1052-1058. Classification revue Q1 “Plant Sciences” IF 2.997
• Le Signor C, Gallardo K, Prosperi JM., Salon C, Quillien L, Thompson R, Duc G (2005) Genetic diversity for seed protein composition in M. truncatula. Plant Genetic Resources: Characterization and Utilization 3: 59-71. Non référencé dans NORIA.
• Rajjou L, Gallardo K, Debeaujon I, Vandekerckhove J, Job C, Job D (2004) The effect of alpha-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination. Plant Physiology 134: 1598-1613. Classification revue Q1 “Plant Sciences” IF 7.089
• Schiltz S, Gallardo K, Huart M, Negroni L, Sommerer N, Burstin J (2004) Proteome reference maps of vegetative tissues in pea. An investigation of nitrogen mobilization from leaves during seed filling. Plant Physiology 135(4): 2241-60. Classification revue Q1 “Plant Sciences” IF 7.089
• Gallardo K, Le Signor C, Vanderkerckhove J, Thompson R, Burstin J (2003) Proteomics of M. truncatula seed development establishes the timeframe of diverse metabolic processes related to reserve accumulation. Plant Physiology 133: 664-682. Classification revue Q1 “Plant Sciences” IF 7.089
• Haslam R.P, Downie A.L, Raveton M, Gallardo K, Job D, Pallett KE, John P, Parry MAJ, Coleman JOD (2003) The assessment of enriched apoplastic extracts using proteomic approaches. Annals of Applied Biology 143: 81-91. Classification revue Q1 “Agriculture, Multidisciplinary” IF 2.26
• Gallardo K, Job C, Groot S.P.C, Puype M, Demol H, Vandekerckhove J, Job D (2002a) Proteomics of Arabidopsis seed germination. A comparative study of wild-type and gibberellin-deficient seeds. Plant Physiology 129: 823-837. Classification revue Q1 “Plant Sciences” IF 7.089
• Gallardo K, Job C, Groot S.P.C, Puype M, Demol H, Vandekerckhove J, Job D (2002b) Importance of methionine biosynthesis for Arabidopsis seed germination and seedling growth. Physiologia Plantarum 116: 238-247. Classification revue Q1 “Plant Sciences” IF 3,473
• Gallardo K, Job C, Groot S.P.C, Puype M, Demol H, Vandekerckhove J, Job D (2001) Proteomic analysis of Arabidopsis seed germination and priming. Plant Physiology 126: 835-848. Classification revue Q1 “Plant Sciences” IF 7.089
• Job C, Laugel S, Duval M, Gallardo K, Job D (2001) Biochemical characterization of atypical biotinylation domains in seed proteins. Seed Science Research 11: 149-161. Classification revue Q2 “Plant Sciences” IF 1.767

Chapitres (ouvrages, magazines)

• Gallardo K, Jolivet P, Vernoud V, Canonge M, Larré C and Chardot T (2016) Storage Cells – Oil and Protein bodies.  In Molecular Cell Biology of the Growth and Differentiation of Plant Cells, CRC Press, Ed. Ray J. Rose. P. 362-381.
• Gallardo K, D'Erfurth I, Le Signor C, Vernoud V, Aubert G, Buitink J, Gouzy J, Prosperi J-M, Burstin J, Thompson R (2015). Use of translational genomics to identify genes important for legume seed development. Legume Perspectives. 8: 12-14.
• Noguero M, Le Signor C, Vernoud V, Aubert G, Sanchez M, Gallardo K, Verdier J, Thompson R (2015). Regulation of legume seed size by an endosperm-expressed transcription factor. Legume Perspectives. 7:12-13.
• Noguero M, Gallardo K, Verdier J, Le Signor C, Burstin J, Thompson R (2013) Legume Seed Genomics: How to Respond to the Challenges and Potential of a Key Plant Family? In Seed Genomics, Wiley & Sons, Ed. Becraft PW, p. 179-201.
• Repetto O and Gallardo K (2012) Organelle proteomics of developing seeds: comparison with other plant tissue organelles. In Seed development. Omics Technologies toward Improvement of Seed Quality and Crop Yield. Part V: Towards SYSTEMS BIOLOGY: ORGANIZATION, INTEGRATION AND MODELIZATION OF DATA, Springer publisher, Eds. GK. Agrawal & R. Rakwal, p. 213-246.
• Zuber H, Noguero M, Le Signor C, Thompson R, Gallardo K (2012) Metabolic specialization of maternal and filial tissues. In Seed development. Omics Technologies toward Improvement of Seed Quality and Crop Yield. Part V: Towards SYSTEMS BIOLOGY: ORGANIZATION, INTEGRATION AND MODELIZATION OF DATA, Springer publisher, Eds. GK. Agrawal & R. Rakwal, p. 407-432.
• Burstin J, Gallardo K, Mir R.R, Varshney R.K, Duc G (2011) Improving Protein Content and Nutrition Quality (Eds Pratap A and Kumar J) CAB International. In Biology and Breeding of Food Legumes, p. 314-328.
• Gallardo K, Junker B.H. & Teulat-Merah B (2011) Exploiting model species for studies of legume seed biology. Grain Legumes Magazine 55.
• Zuber H, Aubert G, Davidian JC, Thompson R and Gallardo K (2009) Sulphur metabolism and transport in developing seeds. (Proceeding) In Sulphur Metabolism in Plants Regulatory Aspects Significance of Sulfur in the Food Chain, Agriculture and the Environment (Eds: A. Sirko, L.J. De Kok, S. Haneklaus, M.J. Hawkesford, H. Rennenber, K. Saito, E Schnug, I Stulen) Margraf Publishers, p. 113-117.
• Gallardo K, Le Signor C, Thompson R, Burstin J (2009) Proteomics in model legume species: a case study in seeds, Grain Legumes Magazine 53.
• Thompson R, Aubert G, Duc G, Gallardo K, Le Signor C (2009) Medicago truncatula as a model legume, Grain Legumes Magazine 53.
• Gallardo K, Burstin J, Thompson R (2006) Unravelling metabolic and transcriptional control of legume seed development. Grain Legumes Magazine 46.
• Gallardo K, Le Signor C, Darmency M, Burstin J, Thompson R; and Rochat C, Boutin JP ; and Kuester H; and Buitink J, Leprince O, Limami A; and Grusak MA (2006) Seed Biology of Medicago truncatula, In: The Medicago truncatula handbook. http://www.noble.org/MedicagoHandbook/
• Nagaraj S, Lei Z, Watson B, Sumner L, Gallardo K, Dumas-Gaudot E, Recorbet G, Robert F, Thiery O, Valot B, Mathesius U, Triplett E (2006) Proteomics, In: The Medicago truncatula handbook. http://www.noble.org/MedicagoHandbook/
• Rajjou L, Gallardo K, Job C, Job D (2006) Proteome Analysis for the Study of Developmental Processes in Plants. In Plant Proteomics. Annual Plant Reviews 28. (Ed. Finnie C) Blackwell Publishing, p. 151-184.
• Gallardo K, Job C, Groot SPC, Puype M, Demol H, Vandekerckhove J, and Job D. Proteomics of Arabidopsis seed germination and priming (2003). (Eds Nicolás G, Bradford KJ, Côme D and Pritchard HW). In The Biology of Seeds: Recent Research Advances; CAB International, p. 199-210.

Vulgarizations:

• Gallardo K, Le Signor C, Thompson R, Burstin J (2009) Proteomics in model legume species: a case study in seeds, Grain Legumes Magazine 53, p.16.
• Thompson R, Aubert G, Duc G,Gallardo K, Lesignor C (2009)Medicago truncatulaas a model legume, Grain Legumes Magazine 53, p.5. 
• Gallardo K, Burstin J, Thompson R (2006) Unravelling metabolic and transcriptional control of legume seed development. Grain Legumes Magazine 46.